siRNA (small interference RNA) 19-22 nt double-stranded RNA. It can knock down the expression of its corresponding gene. has been observed in plant, C.elegans and Drosophila for years. siRNA interference was recently discovered to work in mammalian system. siRNA interference has evolved into a powerful tool to study gene functions.

chemically synthesized siRNA is not very stable, it has to be protected during shipping and de-protected before use. However, vector based siRNA is very stable and can be easily transfected into cell using routine DNA transfection reagents. It also more effective than synthetic siRNA for inhibition of gene expression. Vector based siRNA still has other advantages compared with chemically synthesized siRNA.

Service Overview:
Customer
1) use software to identify siRNA target sites and to design the insert(s);
2) email us the sequence of the designed DNA insert;
3) ship us commercially available siRNA vector.

Biomatik Corp.
1) synthesize the DNA sequence insert;
2) clone the insert into the vector you desire;
3) verify the insert by sequencing.

Delivery Includes:
1- 10ug or 100ug sequence verified plasmid;
2 -Sequencing data.

Delivery Includes:
- 10ug of lyophilized plasmid DNA containing the requested construct with desired mutations     incorporated.
- sequencing data;
- Quality control files.

Discount available for large sequencing project.
Typical turnaround: 5 business days.
Delivery Form: Sequence chromatograms is sent by email.

Sequencing samples are run on ABI 3730 DNA Analyzer. ABI 3730 uses a four-color dye system to provide 600-900 bases usable sequence data for each reaction. which allows maximum flexibility for reading length and sample numbers. High throughput sequencing service of ABI 3730 DNA Analyzer would be helpful for researchers who want to scale up their projects.

Sequencing samples are run on ABI 3730 DNA Analyzer. ABI 3730 uses a four-color dye system, provides 600-900 bases usable sequence data for each reaction which allows maximum flexibility for reading length and sample numbers. High throughput sequencing service of ABI 3730 DNA Analyzer would be helpful for researchers who want to scale up their projects.

Key Features

- Samples QC by agarose;
- Fluorescent dye-terminator sequencing;
- Free universal primers;
-Template preparation and primer synthesis available.

Sample Requirements

Purified PCR products -
Purified by qualified PCR purification kits. One band should appear on agarose gel. Sample volume should be around 10µl in dd-water at a desired concentration of 20ng/µl. Primers should be at 5-10µl in volume.

Un-purified PCR products -
At least 3µg. Primers should be at 5-10µM in concentration and 20-50ul in Volume.

Purified plasmid DNA -
Samples shall be purified by qualified plasmid DNA purification kits. Sample volume should be 30-50µl in dd-water at a desired concentration of 100ng-200ng/µl. Total 5µg-10µg of DNA is required per each sequencing reaction. TE is not recommended to dissolve DNA samples.

Bacterial Samples -
LB Stabs; 1ml of overnight culture containing 15% of glycerol.
Please note that low copy of bacterial stabs or culture are not acceptable. Please send 10µg of purified DNA. Please provide all necessary information including insert size and antibiotic resistance.

Primers -
Universal primers are free of charge. Non-universal primers can be synthesized by Biomtik at extra cost.